Frequently Asked Questions
1. What is RNA sequencing (RNA-Seq)?
RNA-Seq is a next-generation sequencing (NGS) technique used to analyze the transcriptome of organisms. Its digital data, that replaces the analog micro-array based expression analysis and provides comprehensive insights into gene expression levels, transcript isoforms, and novel RNA discovery.
2. What types of RNA are analyzed in our service?
Although, currently through our self-service online seqpro portal we are offering mRNA library prep (poly A capture) and sequencing, our full-service offering includes small RNA, Total RNA or long-non coding RNA sequencing. Please reach out to support@seqpro.ai for our full-service offerings.
3. Where is sequencing done?
At our Foster City lab in California, USA.
4. What types of samples do you accept?
We currently only accept Total RNA with concentration >10ng/µl with RIN>7 and a minimum of 500 ng total RNA in 50 ul for each sample through our online ordering system. For low concentration samples, lower quality samples or need RNA extraction, please reach out to support@seqpro.ai for our full-service offerings.
5. What is included in your RNA-Seq service?
Our RNA-Seq service includes sample QC, mRNA library preparation, library QC, Illumina NovaSeq PE150 sequencing, and data QC report. We can also perform fee-based bioinformatics analysis – please contact us at support@seqpro.ai
6. Are you offering any other service?
For other services, please ‘contact us’.
7. How will the data be delivered?
Data will be delivered via a secure FTP link. Credentials will be shared through client portal. If you need AWS transfer, please reach out to support@seqpro.ai.
8. What is your turnaround time?
3-4 weeks from the sample receipt.
9. What quality and quantity of RNA are required?
We require RNA (RIN > 7, 260/280 >1.8) and a minimum of 500 ng total RNA in 50 ul for each sample.
10. What sequencing platforms do you use?
Illumina NovaSeq X+ .
11. What is your sequencing depth and read length?
Sequencing depth can be chosen during the order process. 10M paired end (20M total) is our suggested offering and is sufficient for expression, differential gene expression and most other analysis where the reference or high quality annotated genomes exist. Higher sequencing depths are available if required. Select your preferred sequencing depth during order placement.
Read length is 2 x 150bp (PE150).
12. Do you provide bioinformatics support?
13. How should I prepare and ship my RNA samples?
RNA samples should be reconstituted in RNase-free water and shipped to us in RNase-free tubes on dry ice. Detailed shipping instructions will be provided once you place the order through our web-portal.
14. Can you work with degraded or low-input RNA samples?
Yes, but only as part of our Full-service Platinum offering. The Self-service Gold offering does not cover these sample types. Please reach out to support@seqpro.ai to place your order.
15. How do I get started with my project?
Please register on our portal and follow the instructions.
16. What happens if my sample fails QC?
If your sample fails QC, you will be charged for the QC. The failed samples will be excluded from the library preparation steps, while all passing samples will proceed through the process. If you prepaid for the service, a credit will be added to your account and applied to your future orders. Please create a new order to submit replacement samples that have failed QC.
17. What payment types are accepted?
Credit Card and Purchase Orders are both accepted.